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Mamani-Matsuda3, M. Degueil2, B. Bennetau2, D. Moynet3, D. Mossalayi3, L. Vellutini2, J.

Pillot2, C. Dejous1, D.

Anti-okadaic acid antibodies were grafted onto the sensor surface. Antibodies, saturating agent, and target species, okadaic acid test line or an irrelevant peptide control line , were injected through an optimized microfluidic chip.


Similarly to sandwich ELISA, polyclonal antibodies were then injected a second time to bind to the acid. They led to frequency shifts three times superior with okadaic acid than with the peptide. Keywords: Acoustic wave; Phycotoxin; Microfluidic; Biodetection 1. Introduction Over the last decades, surface acoustic wave devices have received a great attention for real-time biosensing of cells, bacteria and biomolecules [1]. In particular, Love wave platforms offer high potentialities in terms of mass loading sensitivity in liquid media.

QCM-z500 01

The aim is to demonstrate Love wave sensor performance to detect specific phycotoxin such as okadaic acid, responsible of poisoning Diarrheic Shellfish Poisoning, DSP due to excess amount in shellfish food.

Fournel Tel.

E-mail address: fabien. Love wave sensors Thanks to shear horizontal polarization, Love wave devices have been used as sensors in liquid media.

These guided waves propagate in a thin layer rigidly linked to a piezoelectric substrate. The controlled thickness layer permits to trap the acoustic wave energy near the free sensing surface, thus improving sensitivity to surface variations. Love wave sensor platform is composed of AT-cut Quartz substrate, Interdigital Transducers IDTs as input and output ports of the Love wave delay-line, Si02 guiding layer and a immuno-sensitive coating.

IDTs consisted of 44 pairs of split-fingers A.

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The resulting synchronous frequency of the Love wave platform was near MHz. You can enter several keywords and you can refine them whenever you want.

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Raimbault et al, High frequency microrheological measurements of PDMS fluids using saw microfluidic system, Sensors and Actuators B, , Christus est natus ex Maria virgine.

The electrodes provide alternating current that causes the crystal to move due to the piezoelectric effect inherent in quartz crystals. Turn on the power of the main electronics unit and wait for at least 1 minute or until the light on the front panel stops blinking.

The temperature can be either set manually from the Temperature Control Unit figure 1.

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